解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1014060113186
更新日期:2001-07-01 00:00:00
abstract::The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1014008129116
更新日期:2001-07-01 00:00:00
abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1013192017048
更新日期:2001-05-01 00:00:00
abstract::Both embryonic and somatic stem cells have been studied in recent years with particular regard to their differentiation potential. In vitro studies allow a considerable amplification of such cells in culture as well as the induction of commitment in different directions under proper stimulating factors. Moreover, a su...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1017526408890
更新日期:2001-03-01 00:00:00
abstract::Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008139412588
更新日期:2001-01-01 00:00:00
abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008187310534
更新日期:2000-11-01 00:00:00
abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008154615643
更新日期:2000-10-01 00:00:00
abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008159912634
更新日期:2000-10-01 00:00:00
abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008175226819
更新日期:2000-07-01 00:00:00
abstract::The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008181600197
更新日期:2000-07-01 00:00:00
abstract::Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008141918852
更新日期:2000-07-01 00:00:00
abstract::The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008149815908
更新日期:2000-03-01 00:00:00
abstract::The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008166310368
更新日期:2000-02-01 00:00:00
abstract::The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060502532
更新日期:1999-11-01 00:00:00
abstract::The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008051618059
更新日期:1999-09-01 00:00:00
abstract::To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008048928053
更新日期:1999-09-01 00:00:00
abstract::Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008084508489
更新日期:1999-09-01 00:00:00
abstract::With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008038515285
更新日期:1999-07-01 00:00:00
abstract::Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008005200711
更新日期:1999-07-01 00:00:00
abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008004618163
更新日期:1999-05-01 00:00:00
abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008068718392
更新日期:1999-03-01 00:00:00
abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008021428969
更新日期:1998-11-01 00:00:00
abstract::Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008054403470
更新日期:1998-11-01 00:00:00
abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060720608
更新日期:1998-09-01 00:00:00
abstract::Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008058031511
更新日期:1998-09-01 00:00:00
abstract::The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007942619463
更新日期:1998-03-01 00:00:00
abstract::A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007924119018
更新日期:1997-11-01 00:00:00
abstract::Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007928203997
更新日期:1997-11-01 00:00:00
abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007945826228
更新日期:1997-07-01 00:00:00
abstract::Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007919921991
更新日期:1997-01-01 00:00:00
abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007903315687
更新日期:1997-01-01 00:00:00
abstract::A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02215660
更新日期:1996-06-01 00:00:00
abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744216
更新日期:1996-01-01 00:00:00
abstract:CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350384
更新日期:1996-01-01 00:00:00
abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350396
更新日期:1996-01-01 00:00:00
abstract::This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365341
更新日期:1996-01-01 00:00:00
abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353919
更新日期:1996-01-01 00:00:00
abstract::Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353929
更新日期:1996-01-01 00:00:00
abstract::The CHO cell line has achieved considerable commercial importance as a vehicle for the production of human therapeutic proteins, but is known to lack a functional copy of the gene coding for α2,6-sialyltransferase (EC 2.4.99.1). The cDNA for rat α2,6-ST was expressed in a recombinant CHO cell line making interferon-γ,...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353939
更新日期:1996-01-01 00:00:00
abstract::The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02215666
更新日期:1996-01-01 00:00:00